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AAT Bioquest

FAQ

Posted on May 9, 2020
How can I isolate red blood cells from plasma?
Cellular ProcessesCellular Structures and Organellescell culturecentrifuge


Posted on February 19, 2020
My NAD(H) assays have all given me poor results for an extended period of time. What am I doing wrong?
AmpliteNAD/NADH RatioNADH AssayNADHStoragecell culture


Posted on February 19, 2020
What are possible reasons for inconsistent Fluo-4 AM loading?
AM esterCalcium dyesGreen Fluorescencecalciumcalcium indicatorcell linescell-permeable probescell culturedegree of labelingfluo-4


Posted on February 19, 2020
How do I improve transfection efficiency?
ControlsDNA Concentration CalculatorDNAStorageTransfectioncell culturedegree of labelinglipid


Posted on February 19, 2020
My cells are dying after transfection-what solutions do you recommend?
Cell AssayCell CytotoxicityCell Membrane IntegrityCell ViabilityControlsDNAProteinTransfectioncell culturecell damagecell death


Posted on December 10, 2019
Why do cells recovered from liquid nitrogen have lower viability?
cell cultureCell Assaylive cell analysis


Posted on September 16, 2019
Why are cells washed?
bufferfluorescence imagingimmunofluorescencelive cell analysisno washcell cultureisoelectric point isotonic


Posted on September 6, 2019
What does an assay buffer do?
cell culturecell lysisisotonicbuffer


Posted on August 6, 2019
Assay volumes for various size cell culture plates.
cell culture


Posted on July 9, 2019
What are the differences between diH2O, dH2O, and ddH2O?
Ca2+Mg2+Stock SolutionWorking Solutionbacteriacell culturePurification


Posted on July 8, 2019
What is the difference between PBS and dPBS?
PBScell culturehumandPBSisotonic